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| The 4th ESCCA Clinical Cytometry Course will take place 16-17 September 2008 at the University Bremen, NW2, Bremen, Germany |
| Scientific organizing committee |
| Maria Arroz (chair), Lisbon (PT) Bruno Brando, Milan (IT) Mariam Klouche, Bremen, (DE) Jaco Kraan, Rotterdam, (NL) Stefano Papa, Urbino (IT) |
| How to get to the "University Bremen, NW2" |
University Bremen Click here to see the University Bremen, NW2 location on Google Maps |
HIDE COURSE INTRODUCTION
Introduction to the courses The previous courses have ben very successful and encouraged us to organize an even more flexible Fourth Course with 6 different options aimed to meet the expectations of medical doctors, PhDs, laboratory managers and technical laboratory staff related to flow cytometry. This year the courses are set for two different levels of expertise: Cytometry Toolbox 1 and 2 and Basic Leukemia for beginners and less experienced cytometrists, and Lymphoma, Advanced Leukemia and Myelodysplasia courses for more experienced cytometrists. Both groups will include a theoretical and a practical approach. Lectures will take place in the mornings and cover major topics of interest to each group. Basic Leukemia is recommended for beginners in this field (less than 1 year of experience of analysis, interpretation and reporting of results of leukemic samples). Hands-on will include data file analysis of selected cases, performed by groups of 5 students working with 1 computer and supervised by the Faculty. Cytometry toolbox 1 and 2 are wetlabs. Cytometry Toolbox 1 is aimed at beginners in this field, and will cover instrument set-up, calibration and compensation issues performed on different flow cytometers and Quality Assurance Schemes. Cytometry Toolbox 2 will focus on absolute counts of CD4 T cells and CD34 cells, as well as primary immunodeficiencies and will provide useful advice on how to survive a complicated day in the flow cytometry lab. It is recommended that the attendees should have at least 4 years of experience to apply for the Advanced Course and the Myelodysplasia Course. Each course will include open discussions of previously provided and analysed files (15 minutes/case) followed by comments by the faculty (15 minutes/case). The Myelodysplasia Course will focus on the maturation patterns of both CD34+ cells and CD34- cells in bone marrow. This course is particularly recommended for students who have previously attended the Advanced Leukemia Course. The Lymphoma Course will cover B and T cells proliferation in different samples, including normal, reactive and neoplastic lymph nodes and CSF (at least 2 years of experience are recommended). The fact that the Faculty includes both European and American speakers is also of added value. CME credits will be awarded to each course by the European Hematology Association. Students will be given the presentations and the files of the cases related to the course they attended. |
ESCCA COURSE TIMETABLE |
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Tuesday |
Wednesday |
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Morning |
ESCCA |
ESCCA |
Coffee Break |
10 – 10:30 am
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Noon |
ESCCA |
ESCCA |
Coffee Break |
12 – 1:30 pm |
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Afternoon |
ESCCA |
ESCCA |
Break |
3:30 – 4 pm
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|
Late |
ESCCA |
ESCCA |
Evening |
Opening Industrial Exhib. |
Gala Dinner |
COURSE PROGRAMME |
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| Lectures for Basic Leukemia, Cytometry Toolbox 1 and Cytometry Toolbox 2 | |
Jaco Kraan (Rotterdam, NL) |
Instrument set-up, calibration and measurement of performance |
Claudio Ortolani (Venice, IT) |
Multicolor compensation |
Bruce Greig (Nashville, US) |
Education and training requirements for performing clinical flow cytometry and how to prepare for an inspection? |
Maurice R. G. O’ Goorman (Chicago, US) |
Primary immunodeficiencies |
Bruno Brando (Milan, IT) |
Absolute cell counts – positive/negative analysis |
Elizabeth Stone (Nashville, US) |
Sample processing” and intracellular staining |
David Barnett (Sheffield, UK) |
EQA for immune monitoring |
Judith Chezar (Nahariya,IL) |
“Evaluation of spherocytosis by flow cytometry” and cell cycle analysis |
| Basic Leukemia (maximum 45 participants) Case studies by Elizabeth Stone, Borowitz, Judith Chezar |
| Cytometry Toolbox 1 (maximum 20 participants) Wet lab by Claudio Ortolani, Bruno Brando, David Barnett |
| Cytometry Toolbox 2 (maximum 20 participants) Wet lab by Jaco Kraan, Bruce Greig, Maurice R.G. O’ Goorman |
| Lectures for Basic Leukemia, Cytometry Toolbox 1 and Cytometry Toolbox 2 | |
Alberto Orfao (Salamanca, ES) |
Normal maturation of granulocytic cells and normal maturation of other myeloid lineages |
Julia Almeida (Salamanca, ES) |
Normal maturation of monocytic cells |
Raul Braylan (Gainesville, US) |
Normal and reactive lymph nodes |
Sergio Matarraz (Salamanca, ES) |
Immunophenotype of CD34+ cells in myelodysplasia |
David Barnett (Sheffield, UK) |
EQA for leukemia |
Brent Wood (Seattle, US) |
MRD in AML and diagnosis of Hodgkin’s Lymphoma by flow cytometry |
Margarida Lima (Porto, PT) |
T-cell leukemia/lymphoma |
Maryalice Stetler-Stevenson (Bethesda,US)) |
B cell chronic lymphoproliferative disorders |
Stephen Richards (Leeds, UK) |
PNH by flow cytometry |
Jeannine Holden (Atlanta,US) |
MRD in ALL |
| Advanced Leukemia (maximum 45 participants) Case studies by Wood, Richards, Holden |
| Lymphoma (maximum 45 participants) Case studies by Braylan, Lima, Stetler-Stevenson |
| Myelodysplasia (maximum 45 participants) Case studies by Orfao, Almeida, Matarraz |